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Journal / Genetics / Tessuto-specifici miRNA Expression Profiling in…
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Genetics

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Tessuto-specifici miRNA Expression Profiling in Mouse cuore sezioni mediante ibridazione In Situ

Article DOI: 10.3791/57920-v • 08:22 min • September 15th, 2018
September 15th, 2018 •
1, 2, 3
1Department of Cell and Developmental Biology, University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine, Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Yale School of Medicine

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micro-RNA (Mirna) è brevi e altamente omologhi sequenze di RNA, che serve come regolatori trascrizionali di RNA messaggeri (mRNA). Attuali metodi di rilevazione di miRNA variano nella sensibilità e specificità. Descriviamo un protocollo che unisce l'ibridazione in situ e immunostaining per il rilevamento simultaneo di miRNA e proteina molecole su sezioni di tessuto di cuore di topo.

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Genetica problema 139 espressione di micro-RNA profilatura sonda LNA ibridazione in situ immunostaining ipertrofia cardiaca
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