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Journal / Immunology and Infection / 高通量的高效液氧活化病毒膜融合检测 HIV-1…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究
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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究

Article DOI: 10.3791/58074-v • 07:22 min • August 14th, 2018
August 14th, 2018 •
1,2, 1, 1, 1, 1, 1
1Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology, New Jersey City University

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通过流式细胞术或荧光显微镜检测绿色荧光蛋白的表达, 我们描述了以细胞为基础的 HIV-1 融合报告。它可以用来测试病毒进入的抑制剂 (特别是在融合步骤) 在无细胞和细胞对细胞感染系统。

Tags

免疫学和感染 问题 138 HIV-1 进入 融合 筛选 recombinase 细胞到细胞传输 嘌呤受体
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