Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Isolation of Physiologically Active Thylakoids…
JoVE Journal
Biochemistry

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.

JoVE Journal Biochemistry
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Journal (Biochemistry)
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Journal (Biochemistry)
Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
 

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Article DOI: 10.3791/58393-v • 12:25 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1, 1
1Department of Plant Biology, University of California - Davis

Kapitel

Summary
Automatic Translation

We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.

Tags

Thylakoids Protein Transport Assays Mechanism Energetics Location Of Proteins Control Of Environment Temperature PH Ionic Conditions Precursor Concentrations Peas Distilled Water Plastic Tray Vermiculite Growth Period Chloroplast Isolation Continuous Density Gradient Percoll 2xGB Buffer Centrifugation Pea Shoots 1xGB Buffer Blender Homogenization Filtration Cheese Cloth Centrifuge Tube
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter