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Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
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JoVE Journal Biotechnik
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

DOI:
10.3791/58830-v

•

06:15 min

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January 30, 2019

•

,
1Nanotechnology Characterization Lab., Cancer Research Technology Program,Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute

Kapitel

  • 00:04Titel
  • 00:32Turbidity Limulus Amoebocyte Lysate (LAL) Assay
  • 02:26Sample Loading and Preparation and Gel-Clot LAL
  • 04:27Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
  • 05:12Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Detection of endotoxins in engineered nanomaterials represents one of the grand challenges in the field of nanomedicine. Here, we present a case study that describes the framework composed of three different LAL formats to estimate potential endotoxin contamination in nanoparticles.

Tags

EndotoxinNano-formulationsLimulus Amoebocyte Lysate (LAL) AssaysCalibration StandardControl Standard EndotoxinLAL Grade WaterQuality ControlInstrument SettingsTest IDData GroupHardwareLAL MethodSerial NumberSystem IDSerial PortSample IDNegative ControlStandard CurveTest SamplesInhibition Enhancement ControlDilution

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