Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Identifikation af celleoverflade markører for…
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
Identifikation af celleoverflade markører for primær neurale stamme og stamceller ved metabolisk mærkning af Sialoglycan
Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
Journal (Neuroscience)
Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
Journal (Neuroscience)
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
 
Click here for the English version

Identifikation af celleoverflade markører for primær neurale stamme og stamceller ved metabolisk mærkning af Sialoglycan

Article DOI: 10.3791/58945-v • 11:39 min • September 7th, 2019
September 7th, 2019 •
*1, *2, 1
1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Præsenteret her er en protokol, der kombinerer en in vitro neurale-Endothelial Co-kultur system og metabolisk inkorporering af sialoglycan med bioorthogonal funktionelle grupper til at udvide primære neurale stamme og stamcelle celler og mærke deres overflade sialoglycoproteiner til billeddannelse eller massespektrometri analyse af celleoverflade markører.

Tags

Neurovidenskab metabolisk Les mærkning sialsyrer bioorthogonal reaktion neurale stamceller progenitorceller overflade markører endotelceller proteome kemisk mærkning hjernebarken
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter