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Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
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JoVE Journal Biotechnik
Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

DOI:
10.3791/59123-v

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07:39 min

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January 22, 2019

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,
1Department of Biomedical Engineering,The Ohio State University, 2The Ohio State University Comprehensive Cancer Center,Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department,King Saud University

Kapitel

  • 00:04Titel
  • 00:46Assay Media and Hydrogel Preparation
  • 01:41Encapsulate Cells in Hydrogels
  • 03:51MMP and Metabolic Activity Measurement
  • 05:08Results: Measuring MMP and Metabolic Activities of Cells Cultured in 3D Fluorogenic Hydrogels
  • 06:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, a protocol is presented for encapsulating and culturing cells in poly(ethylene glycol) (PEG) hydrogels functionalized with a fluorogenic matrix metalloproteinase (MMP)-degradable peptide. Cellular MMP and metabolic activity are measured directly from the hydrogel cultures using a standard microplate reader.

Tags

3D HydrogelsCellular FunctionFluorescent SensorProtease ActivityHigh-throughput Drug ScreeningHydrogel PreparationCell EncapsulationA375 Melanoma CellsPBS Buffer96-well Plate

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