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JoVE Journal
Genetics

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A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
 

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Article DOI: 10.3791/59449-v 08:27 min May 22nd, 2019
May 22nd, 2019

Kapitel

Summary

Here, we describe a protocol to generate a viable female murine model with non-random X chromosome inactivation, i.e., the maternally-inherited X chromosome is inactive in 100% of the cells. We also describe a protocol to test feasibility, tolerability, and safety of pharmacological reactivation of the inactive X chromosome in vivo.

Tags

Keywords: Non-random Mouse Model Mecp2 Inactive X Chromosome Xi Reactivation Stereotactic Injection Chemical Inhibitor Brain Neuroscience X-linked Disease Models DDX3X Syndrome
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