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Journal / Cancer Research / Flow Cytometric Analysis of Mitochondrial…
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Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
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Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Article DOI: 10.3791/59593-v • 09:44 min • September 5th, 2019
September 5th, 2019 •
1, 1
1Blood Cell Development and Function Program, Fox Chase Cancer Center

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Summary
Automatic Translation

We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9.

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Flow Cytometric Analysis Mitochondrial Reactive Oxygen Species Hematopoietic Stem Cells Progenitor Cells MLL-AF9 Driven Leukemia ROS Homeostasis Cellular Function And Survival Genetic Manipulation Gene Deletion Or Overexpression Mitochondrial ROS Production Healthy And Malignant Hematopoietic Populations Protogenic Probe Live Cell Analysis Stem Cell Populations
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