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Journal / Genetics / In Vivo Forward Genetic Screen to Identify Novel…
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JoVE Journal Genetics
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Article DOI: 10.3791/59720-v • 10:00 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1, 2, 1
1Department of Biological Sciences, University of Alabama, 2Department of Genetics, University of Wisconsin-Madison

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Summary
Automatic Translation

We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.

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In Vivo Forward Genetic Screen Identify Novel Neuroprotective Genes Drosophila Melanogaster Protocol Power Of Forward Genetics Neurodegeneration Unbiased Approach Mammalian Models Disease Conditions Alzheimer's Disease Parkinson's Disease Biological Processes Neuronal Function Transmission Homozygous Flies ENU Mutagenized Collection Drosophila Mutants Second Chromosome Testing Chamber Anesthesia Recovery Time Fly Locomotion
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