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Journal / Developmental Biology / 评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C）…
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Developmental Biology
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JoVE Journal Developmental Biology
评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C） 损伤的反应
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评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C） 损伤的反应

Article DOI: 10.3791/59924-v • 12:59 min • February 15th, 2020
February 15th, 2020 •
*1, *1, *1, 1, 1, 1
1Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India
* These authors contributed equally

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Summary
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该协议演示了从小鼠眼部表面细胞同时检测活原培养物中的活性氧物种（ROS）、活细胞和死细胞。2'，7'-二氯氟乙酸酸酯，碘化钠和Hoechst染色分别用于评估ROS，死细胞和活细胞，然后成像和分析。

Tags

发育生物学，第156期，UV-C，原生眼干细胞，活性氧种类（ROS），2'，7'-二氯氟乙酸（DCFDA），活/死细胞检测，UV-C损伤
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