Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / En semi-High-gennemløb tilpasning af NADH-koblede …
JoVE Journal
Biochemistry

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Biochemistry
En semi-High-gennemløb tilpasning af NADH-koblede ATPase assay til screening små molekyle hæmmere
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Journal (Biochemistry)
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
Journal (Biochemistry)
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
 
Click here for the English version

En semi-High-gennemløb tilpasning af NADH-koblede ATPase assay til screening små molekyle hæmmere

Article DOI: 10.3791/60017-v • 10:28 min • August 17th, 2019
August 17th, 2019 •
1,2, 1,2, 3, 2, 1,2
1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

En nicotinamid-adenin dinucleotid (NADH)-koblet ATPase-analyse er blevet tilpasset til halvhøj gennemløbs screening af små molekyle myosin-hæmmere. Denne kinetiske analyse køres i et 384-brønd mikroplade format med samlet reaktions volumen på kun 20 μL pr. brønd. Platformen bør gælde for stort set alle ADP-producerende enzymer.

Tags

Biokemi ATPase assay NADH fluorescens semi High-gennemløb screening inhiberende konstant myosin
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter