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Journal / Developmental Biology / Geração de organóides endometriais primários…
JoVE Journal
Developmental Biology

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Geração de organóides endometriais primários humanos multicelulares

Article DOI: 10.3791/60384-v • 09:20 min • October 4th, 2019
October 4th, 2019 •
*1, *2, 1, 1, 1
1Department of Obstetrics and Gynecology, Northwestern University, 2National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)
* These authors contributed equally

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Um protocolo para gerar os organóides endometrial preliminares humanos que consistem em pilhas epithelial e stromal e retêm características do tecido endometrial nativo é apresentado. Este protocolo descreve métodos da aquisição uterine do tecido ao processamento histologic de organoids endometrial.

Tags

Biologia do desenvolvimento edição 152 endométrio organóides cultura 3D organiods primários humanos hormônios células epiteliais e estromais
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