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JoVE Journal Biology
Måling av mitokondrie masse og membran potensial i blodkreft stamceller og T-celler ved Flow flowcytometri
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Måling av mitokondrie masse og membran potensial i blodkreft stamceller og T-celler ved Flow flowcytometri

Article DOI: 10.3791/60475-v • 07:57 min • December 26th, 2019
December 26th, 2019 •
*1,2, 3, 1,3, 1, 2,4, *1
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)
* These authors contributed equally

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Her beskriver vi en pålitelig metode for å måle mitokondrie masse og membran potensial i ex vivo kultivert blodkreft stamceller og T-celler.

Tags

Biologi blodkreft stamceller T celler tumor infiltrere lymfocytter mitokondrier mitokondrie membran potensial mitokondrie masse metabolisme
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