Elektromechanische Bewertung der optogenetisch modulierten Kardiomyozytenaktivität
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March 05, 2020
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Wir präsentieren ein Protokoll zur Bewertung der elektromechanischen Wirkungen der GtACR1-Aktivierung in Kaninchen-Kardiomyozyten. Wir bieten detaillierte Informationen über Zellisolation, Kultivierung und adenovirale Transduktion sowie über funktionelle Experimente mit den Patch-Clamp- und Carbonfaser-Techniken.
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Cite this Article
Kopton, R. A., Buchmann, C., Moss, R., Kohl, P., Peyronnet, R., Schneider-Warme, F. Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity. J. Vis. Exp. (157), e60490, doi:10.3791/60490 (2020).
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