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Journal / Genetics / İplik Spesifik RT-PCR ile Hastalarda İnsan İmmün…
JoVE Journal
Genetics

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JoVE Journal Genetics
İplik Spesifik RT-PCR ile Hastalarda İnsan İmmün Yetmezlik Virüs Tip 1 (HIV-1) Antisense Protein (ASP) RNA Transkriptlerinin Saptanması
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İplik Spesifik RT-PCR ile Hastalarda İnsan İmmün Yetmezlik Virüs Tip 1 (HIV-1) Antisense Protein (ASP) RNA Transkriptlerinin Saptanması

Article DOI: 10.3791/60511-v • 08:01 min • November 27th, 2019
November 27th, 2019 •
1, 1, 2, 3, 4, 5, 2, 1, 1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne

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RNA saç tokaları ve döngüler, sekansa özgü astarların yokluğunda ters transkripsiyon (RT) için astar işlevi görebilir ve üst üste gelen antisense transkriptlerin incelenmesine müdahale eder. İplik spesifik RNA'yı tanımlayabilen bir teknik geliştirdik ve bunu HIV-1 antisense protein ASP'yi incelemek için kullandık.

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Genetik Sayı 153 ASP antisense protein HIV-1 kendi kendini astarlama antisense RNA RNA denatürasyonu RT non-spesifik priming antisense transkripsiyon
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