Herstellung von hochtiterinfektiösen Influenza-Pseudotyppartikeln mit Hülllykoproteinen aus hoch pathogenen H5N1- und Avian H7N9-Viren
8,258 Views
•
08:10 min
•
January 15, 2020
DOI:
Summary
Automatically generated
Dieses Protokoll beschreibt einen experimentellen Prozess zur Herstellung hochtiter infektiöser viraler pseudotypisierter Partikel (pp) mit Hüllglykoproteinen aus zwei Influenza-A-Stämmen und wie ihre Infektiosität bestimmt werden kann. Dieses Protokoll ist sehr anpassungsfähig, um pps von jeder anderen Art von umhüllten Viren mit verschiedenen Hüll-Glykoproteinen zu entwickeln.
Verwandte Videos
07:29
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
Verwandte Videos
41496 Views
12:09
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Verwandte Videos
19252 Views
11:08
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
Verwandte Videos
35281 Views
12:00
Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
Verwandte Videos
11154 Views
12:18
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
Verwandte Videos
24072 Views
08:40
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
Verwandte Videos
58919 Views
07:15
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
Verwandte Videos
2220 Views
06:17
Expression and Purification of Virus-like Particles for Vaccination
Verwandte Videos
21858 Views
01:59
A Technique for the Generation of Influenza Virus-Like Particles via a Mammalian System
Verwandte Videos
185 Views
01:28
Generating Recombinant Live-Attenuated Influenza Viruses for Vaccines
Verwandte Videos
117 Views
Read Article
Cite this Article
Zhang, F., Wang, Y., Shang, X., Wang, S., Xiao, R., Zhou, H., Cai, L. Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses. J. Vis. Exp. (155), e60663, doi:10.3791/60663 (2020).
Copy