Isolierung und Charakterisierung von Exosomen aus Skelettmuskelfibroblasten
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May 16, 2020
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Dieses Protokoll veranschaulicht 1) die Isolierung und Kultivierung von primären Fibroblasten aus dem adulten Musculus gastrocnemius der Maus sowie 2) die Aufreinigung und Charakterisierung von Exosomen unter Verwendung einer differentiellen Ultrazentrifugationsmethode in Kombination mit Saccharosedichtegradienten gefolgt von Western-Blot-Analysen.
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van de Vlekkert, D., Qiu, X., Annunziata, I., d'Azzo, A. Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts. J. Vis. Exp. (159), e61127, doi:10.3791/61127 (2020).
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