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Journal / Bioengineering / 人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成…
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Bioengineering

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JoVE Journal Bioengineering
人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成
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人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成

Article DOI: 10.3791/61143-v • 10:40 min • June 13th, 2020
June 13th, 2020 •
1,2, 1, 1, 1, 1,2,3, 4, 4, 4, 2, 1
1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA

Kapitel

Summary
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该协议描述了一个动态培养系统，用于利用一种一种用途的生物反应器，产生控制大小的人类多能干细胞，进一步刺激在化学定义和免饲料条件下小脑器官的分化。

Tags

生物工程，第160期，人类诱导多能干细胞，小脑分化，动态系统，明确的培养条件，冷冻，免疫污染
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