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Journal / Bioengineering / Preparation of Mesh-Shaped Engineered Cardiac…
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Bioengineering

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Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Article DOI: 10.3791/61246-v • 05:05 min • June 9th, 2020
June 9th, 2020 •
1,2,6, 1,2, 1, 3, 4,5,7, 1,2
1Clinical Translational Research Program, RIKEN Center for Biosystems Dynamics Research, 2Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, 3Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Developmental Biology Center, National Institutes of Health, 4Kosair Charities Pediatric Heart Research Program, Cardiovascular Innovation Institute, University of Louisville, 5Department of Pediatrics, School of Medicine, University of Louisville, 6Department of Cardiovascular Surgery, Mitsubishi Kyoto Hospital, 7Cincinnati Children's Heart Institute

Summary
Automatic Translation

The present protocol generates mesh-shaped engineered cardiac tissues containing cardiovascular cells derived from human induced pluripotent stem cells to allow the investigation of cell implantation therapy for heart diseases.

Tags

Mesh-shaped Engineered Cardiac Tissues Human IPS Cells Myocardial Repair Tissue Geometry Tissue Function Rat Myocardial Infarction Model Cardiac Regenerative Therapy PDMS Sheet Silicone Adhesive Rectangular Tray Rectangular Posts Poloxamer 407 PBS Six-well Plate Lineage Analysis Cardiomyocyte Cells Endothelial Cells Vascular Mural Cells
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