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Journal / Cancer Research / 人类结肠癌细胞DNA损伤与修复福西的免疫荧光成像…
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Cancer Research

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人类结肠癌细胞DNA损伤与修复福西的免疫荧光成像

Article DOI: 10.3791/61399-v • 05:18 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

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Summary
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在中子捕获治疗（BNCT）中使用的中子混合束激活的辐射诱导DNA损伤反应尚未完全确定。该协议提供了一个分步程序，通过使用中子混合束照射后，通过人体结肠癌细胞系中的免疫荧光染色来检测修复蛋白质的辐射诱导 foci （RIF）。

Tags

癌症研究， 问题 160， BNCT， DNA-DSB， 辐射诱导的病源， DNA损伤， 修复途径， 混合光束
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