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Journal / Developmental Biology / Un protocolo para la inmunohistoquímica y la…
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Developmental Biology
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JoVE Journal Developmental Biology
Un protocolo para la inmunohistoquímica y la distribución in situ de ARN dentro del embrión temprano de Drosophila
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Un protocolo para la inmunohistoquímica y la distribución in situ de ARN dentro del embrión temprano de Drosophila

Article DOI: 10.3791/61776-v • 14:34 min • May 6th, 2022
May 6th, 2022 •
*1, *1, *2,3, 1, 1, 1, 1, 1, 1, 1,4, 1, 5, 6, 7, 3, 1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
* These authors contributed equally

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Aquí, describimos un protocolo para la detección y localización de la proteína embrionaria de Drosophila y el ARN desde la recolección hasta la preincrustación e incrustación, la inmunotinción y la hibridación in situ de ARNm.

Tags

Biología del desarrollo Número 183 Embrión de Drosophila sección de parafina portaobjetos de recubrimiento incrustación hibridación in situ DmFKBP12/Calstabina
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