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Neuroscience

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JoVE Journal Neuroscience
Generering av humane nevroner og oligodendrocytter fra pluripotente stamceller for modellering av neuron-oligodendrococyttinteraksjoner
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Generering av humane nevroner og oligodendrocytter fra pluripotente stamceller for modellering av neuron-oligodendrococyttinteraksjoner

Article DOI: 10.3791/61778-v • 10:53 min • November 9th, 2020
November 9th, 2020 •
*1, *1,2, *3, 1, 1, 3, 3,4, 1,5,6
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University
* These authors contributed equally

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Neuron-glial-interaksjonene i nevrodegenerasjon er ikke godt forstått på grunn av utilstrekkelige verktøy og metoder. Her beskriver vi optimaliserte protokoller for å oppnå induserte nevroner, oligodendrocytter forløperceller og oligodendrocytter fra humane pluripotente stamceller og gir eksempler på verdiene av disse metodene for å forstå celletypespesifikke bidrag i Alzheimers sykdom.

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Nevrovitenskap utgave 165 induserte pluripotente stamceller iPS-celler humane embryonale stamceller ES-celler nevroner oligodendrocytter oligodendrocytter oligodendrocytter OPC Alzheimers sykdom amyloid-beta-peptider synapser
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