Entwicklung und Prüfung artspezifischer quantitativer PCR-Assays für Umwelt-DNA-Anwendungen
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November 05, 2020
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Umwelt-DNA-Assays erfordern eine strenge Konzeption, Prüfung, Optimierung und Validierung, bevor mit der Erfassung von Felddaten begonnen werden kann. Hier stellen wir ein Protokoll vor, um Benutzer durch jeden Schritt der Entwicklung eines artspezifischen, sonsonenbasierten qPCR-Tests zum Nachweis und zur Quantifizierung einer Zielart-DNA aus Umweltproben zu führen.
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Cite this Article
Klymus, K. E., Ruiz Ramos, D. V., Thompson, N. L., Richter, C. A. Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications. J. Vis. Exp. (165), e61825, doi:10.3791/61825 (2020).
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