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Journal / Environment / Evaluating the Impact of Hydraulic Fracturing on…
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Environment

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JoVE Journal Environment
Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Article DOI: 10.3791/61904-v • 09:11 min • April 4th, 2021
April 4th, 2021 •
1,2, 1, 1,2, 1, 3, 4,5, 1,2
1Department of Biology, Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences, Michigan Technological University, 4Biosciences Division, Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering, University of Tennessee

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Summary
Automatic Translation

Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.

Tags

Hydraulic Fracturing Streams Microbial Molecular Signatures Impact Evaluation Bacteria Sample Collection Data Analysis Sediment Samples Nucleic Acid Extraction DNA/RNA Preservative Laboratory 16S DNA Analysis Metatranscriptomics RNA Analysis Filter Collection
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