Einzelmolekül-Verweilzeitanalyse der Restriktionsendonuklilease-vermittelten DNA-Spaltung
1,981 Views
•
09:53 min
•
February 07, 2021
DOI:
Summary
Automatically generated
Mit Hilfe von Quantenpunkt-markierter DNA und Totalreflexionsfluoreszenzmikroskopie können wir den Reaktionsmechanismus von Restriktionsendonukleasen untersuchen, während wir unmarkierte Proteine verwenden. Diese Einzelmolekültechnik ermöglicht die massiv gemultiplexte Beobachtung einzelner Protein-DNA-Wechselwirkungen, und Daten können gepoolt werden, um gut bevölkerte Verweilzeitverteilungen zu erzeugen.
Verwandte Videos
14:27
Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Verwandte Videos
19381 Views
08:31
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
Verwandte Videos
9111 Views
06:24
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
Verwandte Videos
8022 Views
09:16
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Verwandte Videos
7301 Views
N/A
DNA Curtains Shed Light on Complex Molecular Systems During Homologous Recombination
Verwandte Videos
2145 Views
17:03
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
Verwandte Videos
18742 Views
07:37
Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
Verwandte Videos
1521 Views
09:53
Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
Verwandte Videos
2.0K Views
06:51
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Verwandte Videos
4.0K Views
09:16
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Verwandte Videos
7.3K Views
Read Article
Cite this Article
Etson, C. M., Todorov, P., Shatery Nejad, N., Shrestha, N., Walt, D. R. Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage. J. Vis. Exp. (168), e62112, doi:10.3791/62112 (2021).
Copy