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Journal / Developmental Biology / 基于2D和3D人诱导多能干细胞的模型，用于剖析新皮质发育过程中原发性纤毛受累…
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Developmental Biology
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JoVE Journal Developmental Biology
基于2D和3D人诱导多能干细胞的模型，用于剖析新皮质发育过程中原发性纤毛受累
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基于2D和3D人诱导多能干细胞的模型，用于剖析新皮质发育过程中原发性纤毛受累

Article DOI: 10.3791/62667-v • 14:19 min • March 25th, 2022
March 25th, 2022 •
*1, *1, 2, 1, 1, 1, 3, 1, 1, 1, 4, 1, 1, 1,5, 1,6, 2, 1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
* These authors contributed equally

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Summary
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我们提出了基于2D和3D人类诱导多能干细胞（hIPSC）的新皮质发育模型的生成和表征的详细方案，以及能够对原发性纤毛（PC）生物发生和功能进行定性和定量分析的补充方法。

Tags

发育生物学，第181期，原代纤毛，人诱导多能干细胞，hIPSC，神经干和祖细胞，人类大脑皮质发育，神经玫瑰花，背前脑类器官，基于2D和3D hIPSC的新皮质发育模型，大脑类器官，声波刺猬，全安装免疫染色，光学清除，光片显微镜
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