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Immunology and Infection

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Humanisert Mediator Release Assay som en avlesning for Allergen Potency

Article DOI: 10.3791/62702-v • 10:22 min • June 29th, 2021
June 29th, 2021 •
1, 1, 1, 1
1Department of Biosciences, University of Salzburg

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Her presenterer vi mediatorutgivelsesanalysen, ved hjelp av en rottebassofil leukemicellelinje transfektert med den menneskelige IgE-reseptoren, for å simulere degranulering av effektorceller som vanligvis observeres i type 1 allergiske reaksjoner. Denne metoden undersøker allergenenes biologiske aktivitet på en svært følsom, reproduserbar og skreddersydd måte.

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Immunologi og infeksjon utgave 172 allergi RBL-analyse allergi allergen mediatorfrigjøring histamin IgE basofiler AIT
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