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Immunology and Infection

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Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Summary
Automatic Translation

Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation.

Tags

Dendritic Cell Development Short Hairpin RNA Gene Knockdown Hematopoietic Stem Cells Progenitor Cell Line In Vitro Immune Cells Transcription Factors DC Development Lentiviral Transduction FLT3 Ligand Beta Estradiol Polybrene Puromycin
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