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Colorazione immunoistochimica fluorescente multiplexata di quattro tipi di cellule immunitarie endometriali in aborto spontaneo ricorrente

Article DOI: 10.3791/62931-v • 05:16 min • August 4th, 2021
August 4th, 2021 •
1, 1,2, 1, 1, 3, 1, 1,4, 1,5, 3, 1
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

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Nonostante i progressi nell'immunoistochimica multiplex e nell'imaging multispettrale, caratterizzare la densità e il raggruppamento delle principali cellule immunitarie contemporaneamente nell'endometrio rimane una sfida. Questo documento descrive un protocollo dettagliato di colorazione multiplex e l'imaging per la localizzazione simultanea di quattro tipi di cellule immunitarie nell'endometrio.

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Biologia Numero 174 Multiplex Fluorescenza Cellule immunitarie Metodi Colorazione
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