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Journal / Bioengineering / Layer Microdissection of Tricuspid Valve Leaflets …
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Bioengineering

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Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification
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Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification

Article DOI: 10.3791/63522-v • 07:34 min • February 10th, 2022
February 10th, 2022 •
1, 1, 1, 1,2
1Biomechanics and Biomaterials Design Laboratory (BBDL), School of Aerospace and Mechanical Engineering, The University of Oklahoma, 2Institute for Biomedical Engineering, Science and Technology (IBEST), The University of Oklahoma

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Summary
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This protocol describes the biaxial mechanical characterization, polarized spatial frequency domain imaging-based collagen quantification, and microdissection of tricuspid valve leaflets. The provided method elucidates how the leaflet layers contribute to the holistic leaflet behaviors.

Tags

Layer Microdissection Tricuspid Valve Leaflets Biaxial Mechanical Characterization Microstructural Quantification Tissue Layers Tissue Microstructure Microdissection And Testing Framework Mechanical Properties Microstructural Properties Heart Valve Leaflets Required Materials Wax Board DI Water Pipette Scalpel Micro Scissors Thin Forceps Curved Forceps Thick Forceps Pins Biaxial Tester Laser Displacement Sensor Ventricularis Side Chorda Insertions Photograph Atrialis Side Affix Tissue To Board Pins For Stretching Tissue Glass Bead Fiducial Markers
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