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JoVE Journal Developmental Biology
Erzeugung von Atemwegs-Epithelzell-Luft-Flüssig-Grenzflächenkulturen aus humanen pluripotenten Stammzellen
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
Journal (Developmental Biology)
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
Journal (Developmental Biology)
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
 
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Erzeugung von Atemwegs-Epithelzell-Luft-Flüssig-Grenzflächenkulturen aus humanen pluripotenten Stammzellen

Article DOI: 10.3791/63882-v • 10:46 min • June 14th, 2022
June 14th, 2022 •
1,2, 2, 3, 2, 2, 3, 1,2, 1,2
1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center

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Jüngste Fortschritte bei humaninduzierten pluripotenten Stammzelldifferenzierungsprotokollen ermöglichen die schrittweise Ableitung organspezifischer Zelltypen. Hier stellen wir detaillierte Schritte zur Erhaltung und Expansion von iPSC-abgeleiteten Atemwegsbasalzellen und deren Differenzierung zu einem mukoziliären Epithel in Luft-Flüssigkeits-Grenzflächenkulturen zur Verfügung.

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Entwicklungsbiologie Heft 184
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