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Journal / Immunology and Infection / Modeller av murine vaginal kolonisering av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Modeller av murine vaginal kolonisering av anaerob dyrket bakterier
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Modeller av murine vaginal kolonisering av anaerob dyrket bakterier

Article DOI: 10.3791/64032-v • 08:53 min • May 25th, 2022
May 25th, 2022 •
1,2,3, 2,3, 2,3, 2,3, 4, 2,3
1Division of Biology and Biomedical Sciences, Washington University School of Medicine, 2Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, 3Glycobiology Research and Training Center, UCSD, 4Department of Pediatrics, Washington University School of Medicine

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Protokollen presenterer en musemodell av vaginal kolonisering med anaerob dyrkede humane vaginale bakterier. Vi fokuserer på Gardnerella vaginalis, mens vi inkluderer forslag til Prevotella bivia og Fusobacterium nucleatum. Denne protokollen kan også brukes som en veiledning for vaginale inokulasjoner og levedyktig gjenoppretting av andre anaerob dyrket bakterier.

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Immunologi og infeksjon utgave 183 Vagina mikrobiom mikrobiota bakterier kolonisering bakteriell vaginose Gardnerella vaginalis Prevotella bivia Fusobacterium nucleatum infeksjon musemodell
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