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Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
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JoVE Journal Biotechnik
Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

DOI:
10.3791/64036-v

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11:16 min

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September 09, 2022

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, , ,
1Joint Department of Biomedical Engineering,University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute,North Carolina State University, 3Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill

Kapitel

  • 00:10Introduction
  • 00:50Making the Macroporous Alginate Scaffolds
  • 05:47Transduction
  • 10:07Ergebnisse
  • 10:51Conclusions

Summary

Automatische Übersetzung

Automatische Übersetzung

Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.

Tags

Dry Macroporous Alginate ScaffoldsDryduxViral TransductionT CellsSpinoculationRetroNectinCalcium-D-gluconateAlginate SolutionLyophilization48-well Plate24-well Plate

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