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Journal / Biochemistry / Leginon을 사용한 스테이지 틸트를 이용한 단일 입자 Cryo-EM 데이터…
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Biochemistry

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Leginon을 사용한 스테이지 틸트를 이용한 단일 입자 Cryo-EM 데이터 수집
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Leginon을 사용한 스테이지 틸트를 이용한 단일 입자 Cryo-EM 데이터 수집

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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본 프로토콜은 Cryo-EM 실험에서 기울어진 단일 입자 데이터 수집을 위한 일반화되고 구현하기 쉬운 체계를 설명합니다. 이러한 절차는 공기-물 계면의 부착으로 인해 우선적인 방향 편향을 겪는 샘플에 대한 고품질 EM 맵을 얻는 데 특히 유용합니다.

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