Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

Matthew B. Murphy; Kyungsoo Kim; Prince J. Kannankeril; Katherine T. Murray

doi:10.3791/64168

Optimierung der transösophagealen Vorhofstimulation zur Beurteilung der Vorhofflimmeranfälligkeit...

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Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

Optimierung der transösophagealen Vorhofstimulation zur Beurteilung der Vorhofflimmeranfälligkeit bei Mäusen

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08:05 min

June 29, 2022

DOI: 10.3791/64168-v

Matthew B. Murphy¹, Kyungsoo Kim¹, Prince J. Kannankeril¹, Katherine T. Murray¹

¹Departments of Medicine, Pediatrics and Pharmacology,Vanderbilt University School of Medicine

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Overview

This protocol describes the optimization of transesophageal atrial pacing to evaluate atrial fibrillation susceptibility in mice. The method allows for repeated testing in the same animal, enhancing the reproducibility of results.

Key Study Components

Research Area

Atrial fibrillation
Electrophysiology
Mouse models

Background

Understanding atrial fibrillation's electrophysiologic properties.
The importance of protocol standardization for experimental reproducibility.
Effects of pacing-induced parasympathetic stimulation on arrhythmias.

Methods Used

Transesophageal atrial pacing
Mice
ECG recording and analysis

Main Results

Optimal settings for pacing were established.
Data showed variations in AF susceptibility across different testing conditions.
Identified factors that could confound results, such as parasympathetic stimulation.

Conclusions

The study presents a refined protocol for assessing atrial fibrillation in mice.
It underlines the significance of meticulous protocol design in electrophysiological research.

Frequently Asked Questions

What is transesophageal atrial pacing?

It is a technique used to induce and study atrial fibrillation in experimental models, particularly in mice.

Why is it essential to optimize experimental parameters?

Optimizing parameters enhances the accuracy and reproducibility of research findings related to atrial fibrillation.

How can parasympathetic stimulation affect results?

It can lead to inadvertent induction of arrhythmias, confounding the interpretation of data regarding atrial fibrillation susceptibility.

What age should mice be for optimal testing?

Mice should be at least 12 weeks old for acquired models and start biweekly pacing at 8 weeks for genetic models.

What measures are taken to analyze atrial fibrillation?

Electrophysiologic parameters and duration of AF episodes are recorded, allowing for comprehensive analysis of susceptibility.

Why is serial testing important?

It allows researchers to assess changes in AF susceptibility over time in the same animal, improving data integrity.

Is this method applicable to genetic studies?

Yes, it is specifically designed to assess both genetic and acquired models of atrial fibrillation.

Das vorliegende Protokoll beschreibt die Optimierung experimenteller Parameter bei der Verwendung der transösophagealen Vorhofstimulation zur Beurteilung der Anfälligkeit für Vorhofflimmern bei Mäusen.

Die programmierte elektrische Stimulation mittels transösophagealer Vorhofstimulation kann elektrophysiologische Eigenschaften und die Anfälligkeit für Vorhofflimmern bei Mäusen bestimmen. Hier haben wir ein standardisiertes Vorgehen zur Protokollentwicklung beschrieben, das die Reproduzierbarkeit optimieren kann. Der transösophageale Ansatz für die atriale Stimulation ist typischerweise ein Überlebensverfahren, wodurch serielle Tests am selben Tier möglich sind.

Wichtige Komponenten für die Protokollentwicklung sind erste Palettenstudien zur Optimierung experimenteller Parameter für das untersuchte Modell und die Identifizierung von Arrhythmien, die durch Pacing-induzierte parasympathische Stimulation verursacht werden. Für genetische Modelle, bei denen Mäuse ab einem Alter von acht Wochen einer zweiwöchentlichen Vorhofflimmer-Pacing unterzogen werden, um die optimale Periode der AF-Anfälligkeit zu bestimmen, untersuchen Sie beide Geschlechter, da man möglicherweise keinen AF-Phänotyp entwickelt. Führen Sie für erworbene Modelle das Tempo durch, nachdem Mäuse die körperliche Reife erreicht haben.

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