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Reconstitution of Actin-Based Motility with Commercially Available Proteins
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Reconstitution of Actin-Based Motility with Commercially Available Proteins
DOI:

08:40 min

October 28, 2022

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Kapitel

  • 00:04Introduction
  • 01:05Preparation of Protein Solutions
  • 02:04Measurement of Protein Concentrations
  • 04:15Coating of Beads
  • 05:32Preparing the Motility Mix, Slides for Observation, and Microscopy Observation
  • 06:39Results: Measuring Protein Concentrations, Analyzing Comet Formation and Bead Velocity
  • 07:53Conclusion

Summary

Automatische Übersetzung

This protocol describes how to produce actin comets on the surfaces of beads using commercially available protein ingredients. Such systems mimic the protrusive structures found in cells, and can be used to examine physiological mechanisms of force production in a simplified way.

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