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Kapitel
- 00:04Introduction
- 01:05Preparation of Protein Solutions
- 02:04Measurement of Protein Concentrations
- 04:15Coating of Beads
- 05:32Preparing the Motility Mix, Slides for Observation, and Microscopy Observation
- 06:39Results: Measuring Protein Concentrations, Analyzing Comet Formation and Bead Velocity
- 07:53Conclusion
This protocol describes how to produce actin comets on the surfaces of beads using commercially available protein ingredients. Such systems mimic the protrusive structures found in cells, and can be used to examine physiological mechanisms of force production in a simplified way.
