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Immunologie und Infektion
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Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

DOI:
10.3791/64341-v

•

08:17 min

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October 28, 2022

•

, , , , ,
1Department of Biological Sciences,Texas Tech University

Kapitel

  • 00:05Introduction
  • 01:12Cytotoxicity Assay
  • 06:24Results: Tyrode s Buffer Sensitivity to L. major Proteins Compared to M199 and MEK Pathway Activation in L. major
  • 07:40Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Presented here is a protocol using Leishmania major promastigotes to determine the binding, cytotoxicity, and signaling induced by pore-forming toxins. A proof-of-concept with streptolysin O is provided. Other toxins can also be used to leverage the genetic mutants available in L. major to define new mechanisms of toxin resistance.

Tags

Leishmania MajorPore-forming ToxinsCytotoxicity AssayMembrane-perturbing AgentsAmphotericin BPropidium IodideFlow CytometrySerial DilutionPromastigotesMembrane DamageCell Viability

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