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Journal / Bioengineering / In Vitro Cleavage Assays using Purified…
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Bioengineering

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JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
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In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

Article DOI: 10.3791/64392-v • 08:16 min • October 6th, 2022
October 6th, 2022 •
1, 1
1Department of Molecular, Cell and Cancer Biology, UMass Chan Medical School

Kapitel

Summary
Automatic Translation

Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.

Tags

In Vitro Cleavage Assays Purified Recombinant Drosophila Caspases Substrate Screening Initiator Caspase Dronc Putative Substrates High Throughput Screening Caspases From Other Organisms Enzymatic Activity Frozen Tubes Bacterial Cell Lysis Buffer Protease Inhibitors Lysozyme Benzonase
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