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Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Article DOI: 10.3791/64434-v • 12:41 min • December 23rd, 2022
December 23rd, 2022 •
1, 2, 1, 1, 1, 1,3, 2, 1,4, 1,4, 1,5, 1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp

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Summary
Automatic Translation

This protocol describes a semi-automated method for medium- to high-throughput organoid drug screenings and microscope-agnostic, automated image analysis software to quantify and visualize multiparametric, single-organoid drug responses to capture intratumor heterogeneity.

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Multiparametric Tumor Organoid Drug Screening Widefield Live-cell Imaging Bulk Analysis Single-organoid Analysis Analysis Pipeline Translational Relevance Workflow Automated Analysis Pipeline Information Extraction Organoid Screen Clinical Therapy Response Prediction Ex Vivo Organoid Drug Screening Software Solution Platform Agnostic Patient-derived Tumor Organoids (PDTOs) Extracellular Matrix (ECM) Enzymatic Dissociation Microplate Dissociation Enzyme Mechanical Dissociation Incubation
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