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Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture
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JoVE Journal Biotechnik
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

DOI:
10.3791/64554-v

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08:11 min

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October 28, 2022

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,
1Department of Biomedical Engineering,Duke University, 2Departments of Biomedical Engineering, Neurology, and Dermatology,Duke University

Kapitel

  • 00:04Introduction
  • 01:12Drying Hyaluronic Acid (HA)-Norbornene (NB)
  • 02:423D Cell Culture in Microporous Annealed Particle (MAP) Scaffolds
  • 06:03Results: Generating Granular Scaffolds with Controlled Particle Fractions for In Vitro Tissue Engineering
  • 07:32Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Minimizing the variability in the particle fraction within granular scaffolds facilitates reproducible experimentation. This work describes methods for generating granular scaffolds with controlled particle fractions for in vitro tissue engineering applications.

Tags

Particle FractionMicroporous Annealed Particle Scaffolds3D Cell CultureGranular MaterialsMAP ScaffoldsHydrogel ParticlesScaffold PropertiesCell ResponsesWound RepairDrug DeliveryMicrofluidic Microgel GenerationHyaluronic AcidNorborneneMicrogelsLyophilizationPolydimethylsiloxane

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