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Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE

In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

Article DOI: 10.3791/65286-v • 02:03 min • June 23rd, 2023
June 23rd, 2023 •
*1, *1,2,3,4,5
1Board of Governor’s Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, 3Department of Biomedical Sciences, Cedars-Sinai Medical Center, 4Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 5Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles
* These authors contributed equally

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Modellering Hersentumoren In Vivo Elektroporatie-gebaseerde toediening Plasmide-DNA Patiëntmutatiehandtekeningen Tumormodellen Preklinische testen Immunotherapie Muismodel Tumorpopulaties Immuuncelpopulaties Behandelingsrespons Orthotope transplantatie Gevestigde tumorcellijnen Gepersonaliseerde representatie Patiëntspecifieke tumormutaties DNA-constructen Neurale voorlopercellen (NPC's) Mozaïekanalyse met dual-recombinase-gemedieerde cassette-uitwisseling (MADR) Somatische mutagenese Drivermutaties pasgeboren muizenpups delende cellen laterale ventrikels micro-injectie van DNA-plasmiden transposons CRISPR-gericht sgRNA
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