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Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research (Video) | JoVE

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

Article DOI: 10.3791/65618-v • 01:28 min • October 27th, 2023
October 27th, 2023 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 3, 1
1Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Institute of Medicine Nursing, Hubei University of Medicine, 3Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

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Limbal Niche Cells Isolation Identification Limbus Tissue Cell Clusters Embryonic Stem Cell Medium Matrigel Immunofluorescence Real-time Quantitative PCR Flow Cytometry Stem Cell Markers VIM CD90 CD105 PDGFRβ Pan-CK CD73 SCF BMMSCs
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