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Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Article DOI: 10.3791/65804-v • 14:54 min • September 11th, 2023
September 11th, 2023 •
1,2, 1,2, 1,2, 1,2, 2, 2, 2, 3,4, 5,6,7, 8,9, 2, 1,2,10
1Molecular Surgery Laboratory, Stanford University, 2Department of Ophthalmology, Byers Eye Institute, Stanford University, 3Department of Ophthalmology and Visual Neurosciences, University of Minnesota, 4Retina Consultants of Minnesota, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7The Iowa Neuroscience Institute (INI), University of Iowa, 8Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, 9Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, 10Veterans Affairs Palo Alto Health Care System

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Summary
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This protocol presents an integrated biorepository platform for the standardized collection, annotation, and biobanking of high-quality human aqueous humor and vitreous liquid biopsies for molecular downstream analyses, including proteomics, metabolomics, and glycomics.

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Biobanking Human Aqueous Vitreous Liquid Biopsies Molecular Analyses Ocular Tissues Molecular Profiling Ocular Diseases Diagnostic Strategies Therapeutic Strategies Standardized Collection Biobanking Protocol High Quality Samples Data Privacy Compliant Database Coordinate System Sample Retrieval Downstream Experiments Protein Analysis Glycan Analysis Metabolite Analysis Anterior Chamber Paracentesis Needle Aspiration
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