Name: Eine effiziente und schnelle HPLC-QQQ-MS-Methode zur Quantifizierung von Tropanalkaloiden in Heilpflanzen (Video) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
Duration: 9 min 10 s
Description: Erfahren Sie eine schnelle HPLC-QQQ-MS-Methode zur Quantifizierung von Tropanalkaloiden in Anisodus tanguticus. Dieses Protokoll ermöglicht präzise Analyse für Qualitätskontrolle in der Phytochemie.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Ji, P., Yang, X., Zhao, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+metabolomics+in+quality+control+of+traditional+Chinese+medicines%3A+a+review%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Application of metabolomics in quality control of traditional Chinese medicines: a review.</a> Front Plant Sci. 15, 1463666(2024).</li>
<li>Li, S. -S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Phytochemical+variation+among+the+traditional+Chinese+medicine+mu+dan+pi+from+Paeonia+suffruticosa+%28tree+peony%29%5BTitle%5D)+AND+%22Phytochemistry%22%5BJournal%5D)">Phytochemical variation among the traditional Chinese medicine mu dan pi from Paeonia suffruticosa (tree peony).</a> Phytochemistry. 146, 16-24 (2018).</li>
<li>Wang, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pinelliae+rhizoma+alleviated+acute+lung+injury+induced+by+lipopolysaccharide+via+suppressing+endoplasmic+reticulum+stress-mediated+NLRP3+inflammasome%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">Pinelliae rhizoma alleviated acute lung injury induced by lipopolysaccharide via suppressing endoplasmic reticulum stress-mediated NLRP3 inflammasome.</a> Front Pharmacol. 13, 883865(2022).</li>
<li>Pughaih, T., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aPughaih%2C+T+%22Tropane+Alkaloids%3A+Sources%2C+Chemistry%2C+Pharmacology+and+Biotechnology%22">Tropane Alkaloids: Sources, Chemistry, Pharmacology and Biotechnology</a>. , Nature, Springer. Singapore. (2025).</li>
<li>Chen, C., Wang, B., Xiao, Y., Xiong, F., Zhou, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Geographical+origin+discrimination+of+Anisodus+tanguticus+%28Maxim.%29%3A+chemometrics+methods+applied+to+ICP-OES+analysis%5BTitle%5D)+AND+%22Arab+J+Chem%22%5BJournal%5D)">Geographical origin discrimination of Anisodus tanguticus (Maxim.): chemometrics methods applied to ICP-OES analysis.</a> Arab J Chem. 17 (5), 105730(2024).</li>
<li>Cardillo, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Scopolamine%2C+anisodamine+and+hyoscyamine+production+by+Brugmansia+candida+hairy+root+cultures+in+bioreactors%5BTitle%5D)+AND+%22Process+Biochem%22%5BJournal%5D)">Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors.</a> Process Biochem. 45 (9), 1577-1581 (2010).</li>
<li>Yuan, B., Zheng, C., Teng, H., You, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+atropine%2C+anisodamine%2C+and+scopolamine+in+plant+extract+by+nonaqueous+capillary+electrophoresis+coupled+with+electrochemiluminescence+and+electrochemistry+dual+detection%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Simultaneous determination of atropine, anisodamine, and scopolamine in plant extract by nonaqueous capillary electrophoresis coupled with electrochemiluminescence and electrochemistry dual detection.</a> J Chromatogr A. 1217 (1), 171-174 (2010).</li>
<li>Zhang, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+atropine%2C+scopolamine%2C+and+anisodamine+from+Hyoscyamus+niger+L.+in+rat+plasma+by+high-performance+liquid+chromatography+with+tandem+mass+spectrometry+and+its+application+to+a+pharmacokinetics+study%3A+liquid+chromatography%5BTitle%5D)+AND+%22J+Sep+Sci%22%5BJournal%5D)">Simultaneous determination of atropine, scopolamine, and anisodamine from Hyoscyamus niger L. in rat plasma by high-performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetics study: liquid chromatography.</a> J Sep Sci. 37 (19), 2664-2674 (2014).</li>
<li>Chen, C., Wang, B., Li, J., Xiong, F., Zhou, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multivariate+statistical+analysis+of+metabolites+in+Anisodus+tanguticus+%28Maxim.%29+Pascher+to+determine+geographical+origins+and+network+pharmacology%5BTitle%5D)+AND+%22Front+Plant+Sci%22%5BJournal%5D)">Multivariate statistical analysis of metabolites in Anisodus tanguticus (Maxim.) Pascher to determine geographical origins and network pharmacology.</a> Front Plant Sci. 13, 927336(2022).</li>
<li>Chen, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multivariate+statistical+analysis+of+tropane+alkaloids+in+Anisodus+tanguticus+%28Maxim.%29+Pascher+from+different+regions+to+trace+geographical+origins%5BTitle%5D)+AND+%22Acta+Chromatogr%22%5BJournal%5D)">Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins.</a> Acta Chromatogr. 34 (4), 422-429 (2022).</li>
<li>Sawabe, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+determination+of+atropine+and+scopolamine+content+in+Scopolia+extract+powder+by+HPLC%5BTitle%5D)+AND+%22J+Nat+Med%22%5BJournal%5D)">Rapid determination of atropine and scopolamine content in Scopolia extract powder by HPLC.</a> J Nat Med. 65 (2), 395-399 (2011).</li>
<li>Baj, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+HPLC-QQQ-MS%2FMS+and+new+RP-HPLC-DAD+system+utilizing+the+chaotropic+effect+for+determination+of+nicotine+and+its+major+metabolites+cotinine%2Cand+trans-3%E2%80%B2-hydroxycotinine+in+human+plasma+samples%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Application of HPLC-QQQ-MS/MS and new RP-HPLC-DAD system utilizing the chaotropic effect for determination of nicotine and its major metabolites cotinine,and trans-3′-hydroxycotinine in human plasma samples.</a> Molecules. 27 (3), 682(2022).</li>
<li>Chen, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Qualitative+and+quantitative+analysis+of+the+major+constituents+in+Shexiang+tongxin+dropping+pill+by+HPLC-Q-TOF-MS%2FMS+and+UPLC-QqQ-MS%2FMS%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Qualitative and quantitative analysis of the major constituents in Shexiang tongxin dropping pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS.</a> Molecules. 20 (10), 18597-18619 (2015).</li>
<li>Huang, Y. -F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+determination+of+twenty-nine+active+compounds+in+Fuzhengjiedu+granules+by+HPLC-QQQ-MS%2FMS%5BTitle%5D)+AND+%22Heliyon%22%5BJournal%5D)">Simultaneous determination of twenty-nine active compounds in Fuzhengjiedu granules by HPLC-QQQ-MS/MS.</a> Heliyon. 9 (2), e13675(2023).</li>
<li>Zhang, K. -X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improved+quality+control+method+for+prescriptions+of+Polygonum+capitatum+through+simultaneous+determination+of+nine+major+constituents+by+HPLC+coupled+with+triple+quadruple+mass+spectrometry%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Improved quality control method for prescriptions of Polygonum capitatum through simultaneous determination of nine major constituents by HPLC coupled with triple quadruple mass spectrometry.</a> Molecules. 18 (10), 11824-11835 (2013).</li>
<li>Ding, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comprehensive+chemical+profiling+and+quantification+of+Shexiang+xintongning+tablets+by+integrating+liquid+chromatography-mass+spectrometry+and+gas+chromatography-mass+spectrometry%5BTitle%5D)+AND+%22Arab+J+Chem%22%5BJournal%5D)">Comprehensive chemical profiling and quantification of Shexiang xintongning tablets by integrating liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry.</a> Arab J Chem. 16 (3), 104527(2023).</li>
<li>Zhu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+QuEChERS+method+coupled+to+HPLC-QQQ-MS%2FMS+for+the+determination+of+25+banned+pesticide+residues+in+ethnic+medicines%5BTitle%5D)+AND+%22Res+Sq%22%5BJournal%5D)">The QuEChERS method coupled to HPLC-QQQ-MS/MS for the determination of 25 banned pesticide residues in ethnic medicines.</a> Res Sq. , (2022).</li>
<li>Cinelli, M. A., Cline, J., Watson, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applications+of+liquid+chromatography-tandem+mass+spectrometry+in+natural+products+research%3A+Tropane+alkaloids+as+a+case+study%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Applications of liquid chromatography-tandem mass spectrometry in natural products research: Tropane alkaloids as a case study.</a> J Vis Exp. (205), e66620(2024).</li>
<li>Pang, Y., Huang, Y. -K., Li, F., Yang, F. -Q., Xia, Z. -N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+screening+and+evaluation+of+antioxidants+in+alkaloid+natural+products+by+capillary+electrophoresis+with+chemiluminescence+detection%5BTitle%5D)+AND+%22Anal+Methods%22%5BJournal%5D)">Rapid screening and evaluation of antioxidants in alkaloid natural products by capillary electrophoresis with chemiluminescence detection.</a> Anal Methods. 8 (35), 6545-6553 (2016).</li>
<li>Voeten, R. L. C., Ventouri, I. K., Haselberg, R., Somsen, G. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Capillary+electrophoresis%3Atrends+and+recent+advances%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Capillary electrophoresis:trends and recent advances.</a> Anal Chem. 90 (3), 1464-1481 (2018).</li>
<li>Pellati, F., Benvenuti, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chromatographic+and+electrophoretic+methods+for+the+analysis+of+phenetylamine+alkaloids+in+Citrus+aurantium%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Chromatographic and electrophoretic methods for the analysis of phenetylamine alkaloids in Citrus aurantium.</a> J Chromatogr A. 1161 (1), 71-88 (2007).</li>
<li>Cirlini, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+sensitive+UHPLC-ESI-MS%2FMS+method+for+the+determination+of+tropane+alkaloids+in+herbal+teas+and+extracts%5BTitle%5D)+AND+%22Food+Control%22%5BJournal%5D)">A sensitive UHPLC-ESI-MS/MS method for the determination of tropane alkaloids in herbal teas and extracts.</a> Food Control. 105, 285-291 (2019).</li>
<li>Wojakowska, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Structural+analysis+and+profiling+of+phenolic+secondary+metabolites+of+Mexican+lupine+species+using+LC-MS+techniques%5BTitle%5D)+AND+%22Phytochemistry%22%5BJournal%5D)">Structural analysis and profiling of phenolic secondary metabolites of Mexican lupine species using LC-MS techniques.</a> Phytochemistry. 92, 71-86 (2013).</li>
<li>Shimizu, T., Watanabe, M., Fernie, A. R., Tohge, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+LC-MS+analysis+for+plant+secondary+metabolites%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Targeted LC-MS analysis for plant secondary metabolites.</a> Methods Mol Biol. 1778, 171-181 (2018).</li>
<li>Mennickent, S., De Diego, M. Analytical Method Validation as the First Step in Drug Quality Control. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMennickent%2C+S+%22Quality+Management+and+Quality+Control+-+New+Trends+and+Developments%22">Quality Management and Quality Control - New Trends and Developments</a>. , IntechOpen. London, UK. (2019).</li>
<li>Yang, S. H., Wang, J., Zhang, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+a+two-dimensional+liquid+chromatography+method+for+quality+control+testing+of+pharmaceutical+materials%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Validation of a two-dimensional liquid chromatography method for quality control testing of pharmaceutical materials.</a> J Chromatogr A. 1492, 89-97 (2017).</li>
<li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((HPLC+with+chiral+stationary+phase+for+separation+and+kinetics+study+of+aspartic+acid+epimerization+in+peroxiredoxin+2+active+site+peptide%5BTitle%5D)+AND+%22J+Pharm+Biomed+Anal%22%5BJournal%5D)">HPLC with chiral stationary phase for separation and kinetics study of aspartic acid epimerization in peroxiredoxin 2 active site peptide.</a> J Pharm Biomed Anal. 247, 116247(2024).</li>
<li>Lu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolite+measurement%3A+pittfalls+to+avoid+and+practices+to+follow%5BTitle%5D)+AND+%22Annu+Rev+Biochem%22%5BJournal%5D)">Metabolite measurement: pittfalls to avoid and practices to follow.</a> Annu Rev Biochem. 86, 277-304 (2017).</li>
<li>Leopold, J., Popkova, Y., Engel, K. M., Schiller, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+developments+of+useful+MALDI+matrices+for+the+mass+spectrometric+characterization+of+lipids%5BTitle%5D)+AND+%22Biomolecules%22%5BJournal%5D)">Recent developments of useful MALDI matrices for the mass spectrometric characterization of lipids.</a> Biomolecules. 8 (4), 173(2018).</li>
<li>Parasuraman, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+overview+of+liquid+chromatography-mass+spectroscopy+instrumentation%5BTitle%5D)+AND+%22Pharm+Methods%22%5BJournal%5D)">An overview of liquid chromatography-mass spectroscopy instrumentation.</a> Pharm Methods. 5 (2), 47-55 (2014).</li>
<li>Alanazi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+advances+in+liquid+chromatography%E2%80%93mass+spectrometry+%28LC%E2%80%93MS%29+applications+in+biological+and+applied+sciences%5BTitle%5D)+AND+%22Anal+Sci+Adv%22%5BJournal%5D)">Recent advances in liquid chromatography–mass spectrometry (LC–MS) applications in biological and applied sciences.</a> Anal Sci Adv. 6 (1), e70024(2025).</li>
<li>Cortese, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Compensate+for+or+minimize+matrix+effects%3F+Strategies+for+overcoming+matrix+effects+in+liquid+chromatography-mass+spectrometry+technique%3A+A+tutorial+review%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Compensate for or minimize matrix effects? Strategies for overcoming matrix effects in liquid chromatography-mass spectrometry technique: A tutorial review.</a> Molecules. 25 (13), 30-47 (2020).</li>
<li>Wang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+interplay+between+herbal+medicines+and+gut+microbiota+in+metabolic+diseases%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">The interplay between herbal medicines and gut microbiota in metabolic diseases.</a> Front Pharmacol. 14, 1105405(2023).</li>
<li>Musharraf, S. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Screening+of+inhibitors+of+angiotensin-converting+enzyme+%28ACE%29+employing+high+performance+liquid+chromatography-electrospray+ionization+triple+quadrupole+mass+spectrometry+%28HPLC-ESI-QqQ-MS%29%5BTitle%5D)+AND+%22Eur+J+Pharm+Sci%22%5BJournal%5D)">Screening of inhibitors of angiotensin-converting enzyme (ACE) employing high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (HPLC-ESI-QqQ-MS).</a> Eur J Pharm Sci. 101, 182-188 (2017).</li>
<li>Liu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Integrating+qualitative+and+quantitative+assessments+of+Yougui+pill%2C+an+effective+traditional+Chinese+medicine%2C+by+HPLC-LTQ-orbitrap-MSn+and+UPLC-QqQ-MS%2FMS%5BTitle%5D)+AND+%22Anal+Methods%22%5BJournal%5D)">Integrating qualitative and quantitative assessments of Yougui pill, an effective traditional Chinese medicine, by HPLC-LTQ-orbitrap-MSn and UPLC-QqQ-MS/MS.</a> Anal Methods. 9 (23), 3485-3496 (2017).</li>
<li>Parks, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Redirecting+tropane+alkaloid+metabolism+reveals+pyrrolidine+alkaloid+diversity+in+Atropa+belladonna%5BTitle%5D)+AND+%22New+Phytol%22%5BJournal%5D)">Redirecting tropane alkaloid metabolism reveals pyrrolidine alkaloid diversity in Atropa belladonna.</a> New Phytol. 237 (5), 1810-1825 (2023).</li>
<li>Yan, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Triterpenoid+saponins+profiling+by+adducts-targeted+neutral+loss+triggered+enhanced+resolution+and+product+ion+scanning+using+triple+quadrupole+linear+ion+trap+mass+spectrometry%5BTitle%5D)+AND+%22Anal+Chim+Acta%22%5BJournal%5D)">Triterpenoid saponins profiling by adducts-targeted neutral loss triggered enhanced resolution and product ion scanning using triple quadrupole linear ion trap mass spectrometry.</a> Anal Chim Acta. 819, 56-64 (2014).</li>
<li>Sosa-Ferrera, Z., Mahugo-Santana, C., Santana-Rodríguez, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analytical+methodologies+for+the+determination+of+endocrine+disrupting+compounds+in+biological+and+environmental+samples%5BTitle%5D)+AND+%22Biomed+Res+Int%22%5BJournal%5D)">Analytical methodologies for the determination of endocrine disrupting compounds in biological and environmental samples.</a> Biomed Res Int. 2013, 674838(2013).</li>
<li>Heyde, B. J., Barthel, A., Siemens, J., Mulder, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+fast+and+robust+method+for+the+extraction+and+analysis+of+quaternary+alkyl+ammonium+compounds+from+soil+and+sewage+sludge%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">A fast and robust method for the extraction and analysis of quaternary alkyl ammonium compounds from soil and sewage sludge.</a> PLoS One. 15 (8), e0237020(2020).</li>
</ol>

<table><tbody><tr><td>Agilent MassHunter Software</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18-Säule</td><td>Agilent Technologies</td><td>880975-902</td><td>C18-Reversed-Phase-Säule, 4,6 × 250 mm, 5 µm</td></tr><tr><td>Anisodamin-Hydrobromid</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>Ameisensäure</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>Lyophilisator</td><td>EYELA, Tokyo, Japan</td><td>FDU-2110</td><td></td></tr><tr><td>Hochgeschwindigkeits-Universalmühle</td><td>Taisite Instrument Co., Ltd., Tianjin, China</td><td>6010210100</td><td>FW-100, 24.000 U/min, 100 g Kapazität, 80 Mesh</td></tr><tr><td>Hyoscyamin</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>Methanol</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>Mikroporöse Membran 0,22 μm</td><td>Millipore</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8 Wassersystem</td><td>MilliporeSigma, Burlington, MA, USA</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>Skopolamin-Hydrobromid</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>

eine effiziente und schnelle hplc-qqq-ms-methode zur quantifizierung von tropanalkaloiden in heilpflanzen

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

HPLC-QQQ-MS chromatographische Trennung und Identifikation von TAs Ein typisches MRM-Chromatogramm, das aus der Analyse einer Standardmischung und eines A.tanguticus-Wurzelextrakts gewonnen wird, ist in Abbildung 3 und Abbildung 4 dargestellt. Unter den optimierten HPLC-Bedingungen wurden alle drei Zieltropanalkaloide: Hyoscyamin, Anisodamin und Scopolamin, innerhalb von 20 Minuten erfolgreich getren...

Eine effiziente und schnelle HPLC-QQQ-MS-Methode zur Quantifizierung von Tropanalkaloiden in Heilpflanzen

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

an-efficient-rapid-hplc-qqq-ms-method-for-quantitation-tropane

Research

Forschung

JoVE Journal

Chemistry

Chemie

Verfahren zur Identifizierung von Arten von botanischen Material durch direkte Analyse in Echtzeit hochauflösende Massenspektrometrie und multivariate statistische Analyse vorgestellt.

Schnelle Hochdurchsatz-Spezies Identifizierung von Pflanzen von Material mit Direktanalyse in Echtzeit hochauflösender Massenspektrometrie

Hier beschreiben wir eine Hochdurchsatz-Verfahren zur umfassenden Droge-Überwachung, die für die verbesserte Auflösung und Erkennung von großen Platten von Medikamenten, Missbrauch und deren Metabolite mit Qualitätskontrolle auf multisegment-Injektion-Kapillare ermöglicht Elektrophorese-Massenspektrometrie.

High-Throughput und umfassende Droge-Überwachung mittels Massenspektrometrie Multisegment-Injektion-Kapillar-Elektrophorese

Eine präzise und reproduzierbare Methode zur in vivo Nukleoside/Nukleotidquantifizierung in Pflanzen wird hier beschrieben. Diese Methode verwendet eine HPLC-MS/MS.

Pflanzenprobenvorbereitung zur Messung des Nukleosid-/Nukleotidgehalts mit EINER HPLC-MS/MS

Das vorliegende Protokoll beschreibt die Kontamination von Pyrrolizidinalkaloiden (PAs) in Teeproben von PA-produzierenden Unkräutern in Teegärten.

Quelle und Weg der Pyrrolizidinalkaloid-Kontamination in Teeproben

Hier wird eine Headspace-Gaschromatographie-Tandem-Quadrupol-Massenspektrometrie-Methode (HS-GC-MS/MS) beschrieben, die für die Bestimmung von Trimethylamin (TMA) in tierischen Arzneimitteln geeignet ist. Das Protokoll umfasst die Vorbehandlung der Proben, die Headspace-Behandlung, die Analysebedingungen, die methodische Validierung und die Bestimmung der TMA in Arzneimitteln tierischen Ursprungs.

Eine verbesserte Technik zum Nachweis von Trimethylamin in der Tierarzneimittel mittels Headspace-Gaschromatographie-Tandem-Quadrupol-Massenspektrometrie

Wir stellen eine Methode zur schnellen Massenspektrometrie (MS)/Massenspektrometrie (MS)-basierten Annotation und Klassifizierung von Tropanalkaloiden vor, die sowohl für die vorläufige Dereplikation von tropanhaltigen Proben als auch für die Entdeckung neuer Alkaloide für die Isolierung nützlich ist.

Anwendungen der Flüssigchromatographie-Tandem-Massenspektrometrie in der Naturstoffforschung: Tropanalkaloide als Fallstudie

Dieses Protokoll stellt eine validierte Massenspektrometrie mit Flüssigchromatographie, Ionenmobilität und hoher Auflösung vor, um das Vorhandensein von Mutterkornalkaloiden in Lebensmitteln in Übereinstimmung mit der kürzlich veröffentlichten Verordnung (EU) 2023/915 der Kommission zu bestimmen.

Nachweis von regulierten Mutterkornalkaloiden in Lebensmittelmatrizen mittels Flüssigchromatographie-Mobilitätsspektrometrie-Flugzeitspektrometrie

Dieses Protokoll beschreibt die wichtigsten Schritte für die Biokonjugation eines Cysteins erforderlich Proteins an ein Maleimid enthalten, einschließlich Reagenz Reinigung, Reaktionsbedingungen Biokonjugat Reinigung und Charakterisierung Biokonjugat.

Synthese von Protein Biokonjugate<em&gt; über</em&gt; Cystein-maleimid Chemie

Wir beschreiben die katalytische Insertionspolymerisation von 5-Norbornen-2-carbonsäure und 5-Vinyl-2-norbornen zu funktionellen Polymeren mit einer sehr hohen Glasübergangstemperatur.

Ein einfaches und effizientes Protokoll für die katalytische Insertion Polymerisation von Functional Norbornene

Ein detailliertes Verfahren für die Synthese eines 125 I-markierten Azid und die radioaktiven Markierung von dibenzocyclooctyne (DBCO) -Gruppe-konjugierten, 13 nm große Goldnanopartikel einen kupferfreien Klick - Reaktion beschrieben.

Ein optimiertes Protokoll für die effiziente radioaktive Markierung von Gold-Nanopartikeln durch eine Verwendung von<sup&gt; 125</sup&gt; I-markiertem Azide prosthetische Gruppe

Erfahren Sie eine schnelle HPLC-QQQ-MS-Methode zur Quantifizierung von Tropanalkaloiden in Anisodus tanguticus. Dieses Protokoll ermöglicht präzise Analyse für Qualitätskontrolle in der Phytochemie.

Eine effiziente und schnelle HPLC-QQQ-MS-Methode zur Quantifizierung von Tropanalkaloiden in Heilpflanzen

Weitere Videos entdecken