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JoVE Encyclopedia of Experiments
Cancer Research

JoVE Encyclopedia of Experiments

Cancer Research

Video encyclopedias of advanced research experiments for scientists in academia and biotech

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650+ Videos

Filmed at top university labs

Practice and theory for each experiment

Table of Contents

Breast Cancer

22 Videos

89 Minutes

Procedures and techniques

15 Videos

56 Minutes

Orthotopic Injection into the Mammary Fat Pad: Establishing Breast Cancer in Mice
3:40

Orthotopic Injection into the Mammary Fat Pad: Establishing Breast Cancer in Mice

21.7K views

Lymphedema Ultrasonography: A Technique to Measure the Change in Thickness of an Affected Tissue
3:13

Lymphedema Ultrasonography: A Technique to Measure the Change in Thickness of an Affected Tissue

2.7K views

Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse
2:56

Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse

4.8K views

Radical Mastectomy: Surgical Removal of the Entire Mammary Gland from a Mouse to Study Cancer Progression
2:21

Radical Mastectomy: Surgical Removal of the Entire Mammary Gland from a Mouse to Study Cancer Progression

3.5K views

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
3:45

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

4.6K views

zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts
2:25

zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts

1.9K views

Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver
5:15

Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver

6.2K views

India Ink Inflation: A Staining Method to Visualize Tumor Nodules
3:07

India Ink Inflation: A Staining Method to Visualize Tumor Nodules

8.8K views

Spatial Measurement of Tumor Interstitial Fluid Pressure: A Method to Measure the Interstitial Fluid Pressure
3:34

Spatial Measurement of Tumor Interstitial Fluid Pressure: A Method to Measure the Interstitial Fluid Pressure

3.2K views

Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland
3:44

Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland

2.2K views

Mechanical Dissociation: A Method to Obtain Viable Cells from a Tissue
5:26

Mechanical Dissociation: A Method to Obtain Viable Cells from a Tissue

4.1K views

Sample Preparation for Metabolomics: A Method to Prepare Cell Samples for Metabolite Profiling
3:01

Sample Preparation for Metabolomics: A Method to Prepare Cell Samples for Metabolite Profiling

4K views

Tail Vein Injection: A Method to Administer Cancer Cells for Metastatic Studies in a Mouse Model
4:01

Tail Vein Injection: A Method to Administer Cancer Cells for Metastatic Studies in a Mouse Model

11.6K views

Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads
6:41

Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads

1.7K views

Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model
3:46

Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model

44 views

In vitro studies

7 Videos

32 Minutes

Generation of Primary Mammospheres: A Technique to Determine Cell Potency
5:07

Generation of Primary Mammospheres: A Technique to Determine Cell Potency

3K views

Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
3:19

Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro

2.5K views

Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation
3:19

Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation

2.5K views

3D Co-culture with Direct Interaction: Co-culturing Cancer Cells with Monocytes to Study Their Interaction
5:48

3D Co-culture with Direct Interaction: Co-culturing Cancer Cells with Monocytes to Study Their Interaction

3.5K views

Organ Conditioned Medium: Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies
3:01

Organ Conditioned Medium: Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies

2K views

3D Culture Basement Membrane Assay: Culturing Cancer Cells on 3D Basement Membrane Protein Matrix to Study Cell Invasion
4:34

3D Culture Basement Membrane Assay: Culturing Cancer Cells on 3D Basement Membrane Protein Matrix to Study Cell Invasion

2.8K views

Soft Agar Colony Formation Assay: A Method to Test Effects of Novel Compounds on Cancer Cell Proliferation
7:31

Soft Agar Colony Formation Assay: A Method to Test Effects of Novel Compounds on Cancer Cell Proliferation

4.6K views

Lung Cancer

25 Videos

111 Minutes

In vitro study

12 Videos

52 Minutes

In Vitro Phototoxicity Assay: A PDT-based Method to Evaluate the Phototoxic Potential of a Photosensitizer in Lung Cancer Cells
5:08

In Vitro Phototoxicity Assay: A PDT-based Method to Evaluate the Phototoxic Potential of a Photosensitizer in Lung Cancer Cells

3.8K views

Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
4:21

Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients

3.9K views

Cell Cycle Analysis: An Approach to Study Cell Cycle Regulation of miRNA-transfected Lung Cancer Cells
3:34

Cell Cycle Analysis: An Approach to Study Cell Cycle Regulation of miRNA-transfected Lung Cancer Cells

2K views

3D Co-culture of Lung Cancer Cells with CAFs: An In Vitro Model System to Study Tumor Progression
3:36

3D Co-culture of Lung Cancer Cells with CAFs: An In Vitro Model System to Study Tumor Progression

2.5K views

Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells
3:02

Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells

1.3K views

Immunofluorescence Assay: A Method to Identify Tumor Cells Captured on a Medical Wire
4:32

Immunofluorescence Assay: A Method to Identify Tumor Cells Captured on a Medical Wire

1.4K views

3-Dimensional Culture of Lung Carcinoma Cells: A Method To Study Cell-Matrix Interactions
4:45

3-Dimensional Culture of Lung Carcinoma Cells: A Method To Study Cell-Matrix Interactions

1.4K views

Dose Escalation: A Method for Developing Drug Resistance in Cancer Cells
4:17

Dose Escalation: A Method for Developing Drug Resistance in Cancer Cells

1.6K views

RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
5:38

RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells

1.7K views

Colony Formation Assay: Assessing the Efficacy of Anticancer Agents on Colony-Forming Lung Cancer Cells
4:11

Colony Formation Assay: Assessing the Efficacy of Anticancer Agents on Colony-Forming Lung Cancer Cells

3.1K views

miRNA Extraction: A Method to Extract miRNA from Plasma Sample
3:59

miRNA Extraction: A Method to Extract miRNA from Plasma Sample

4.1K views

3D DNA FISH: A Technique to Locate a Specific Gene on a Chromosome
5:29

3D DNA FISH: A Technique to Locate a Specific Gene on a Chromosome

1.5K views

In vivo study

6 Videos

26 Minutes

Adenocarcinoma Engraftment: A Method to Implant Cancer Cells into the Murine Lungs
4:36

Adenocarcinoma Engraftment: A Method to Implant Cancer Cells into the Murine Lungs

2.8K views

Intrabronchial Delivery: A Technique to Administer an Experimental Agent Selectively Into a Mouse Lung
3:18

Intrabronchial Delivery: A Technique to Administer an Experimental Agent Selectively Into a Mouse Lung

1.9K views

FDG Based PET/CT Scan: A Method of Using Radiotracer for Non-invasive Imaging of Tumor Metabolism
5:27

FDG Based PET/CT Scan: A Method of Using Radiotracer for Non-invasive Imaging of Tumor Metabolism

1.9K views

Zebrafish Xenograft Assay: A Method to Assess the Anti-cancer Efficacy of Therapeutics on Tumorigenicity in Zebrafish In Vivo
3:56

Zebrafish Xenograft Assay: A Method to Assess the Anti-cancer Efficacy of Therapeutics on Tumorigenicity in Zebrafish In Vivo

1.9K views

In Vivo Antitumor Efficacy Analysis of PDT: A Technique to Determine the Phototoxic Potential of a Photosensitizer in Tumor Bearing Mice
3:59

In Vivo Antitumor Efficacy Analysis of PDT: A Technique to Determine the Phototoxic Potential of a Photosensitizer in Tumor Bearing Mice

1.7K views

Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume
5:35

Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume

3.5K views

Ex vivo study

7 Videos

32 Minutes

CTC Immunofluorescence Assay : A Technique to Characterize Circulating Tumor Cells
4:05

CTC Immunofluorescence Assay : A Technique to Characterize Circulating Tumor Cells

1.7K views

Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
5:55

Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging

2.6K views

Mouse Lung Preparation for Flow Cytometry: Generating Cell Suspension from Lung Tissue for Flow Cytometric Analysis
3:48

Mouse Lung Preparation for Flow Cytometry: Generating Cell Suspension from Lung Tissue for Flow Cytometric Analysis

10.3K views

Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)
3:39

Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)

3.2K views

Immunohistochemical Staining: A Method for Obtaining Spatial Information from Thin Tissue Sections
4:52

Immunohistochemical Staining: A Method for Obtaining Spatial Information from Thin Tissue Sections

1.5K views

Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
5:33

Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices

1.9K views

Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology
4:17

Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology

3.5K views

Leukemia

26 Videos

105 Minutes

Cell isolation & characterization

16 Videos

62 Minutes

May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
3:12

May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells

13.5K views

T-Cell Enrichment: A Technique to Isolate T-Cells from Mixed Cell Population by Magnetic Separation
3:57

T-Cell Enrichment: A Technique to Isolate T-Cells from Mixed Cell Population by Magnetic Separation

3.5K views

Magnetic-Activated Cell Sorting: A Method to Isolate c-Kit Positive Cells
4:41

Magnetic-Activated Cell Sorting: A Method to Isolate c-Kit Positive Cells

1.5K views

BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
3:24

BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle

2.3K views

Density Gradient Centrifugation: A Method to Isolate CLL Cells from Peripheral Blood
3:40

Density Gradient Centrifugation: A Method to Isolate CLL Cells from Peripheral Blood

1.5K views

Negative Immunomagnetic Selection: A Method to Purify B-cells from Peripheral Blood Mononuclear Cells
3:06

Negative Immunomagnetic Selection: A Method to Purify B-cells from Peripheral Blood Mononuclear Cells

1.3K views

Leukemic Subpopulation Harvest: A Method for Spatial Separation of Leukemic Cell Subpopulations from 2D Co-culture
3:44

Leukemic Subpopulation Harvest: A Method for Spatial Separation of Leukemic Cell Subpopulations from 2D Co-culture

1.1K views

G-10 Column Based Leukemia Cell Sorting: A Method to Purify Acute Lymphoblastic Leukemia Cells from Bone Marrow Stromal Cells
2:57

G-10 Column Based Leukemia Cell Sorting: A Method to Purify Acute Lymphoblastic Leukemia Cells from Bone Marrow Stromal Cells

1K views

Retro-Orbital Blood Sampling: A Method for Isolating Mononuclear Cells from the Retro-Orbital Sinus of a Mouse
3:18

Retro-Orbital Blood Sampling: A Method for Isolating Mononuclear Cells from the Retro-Orbital Sinus of a Mouse

9.6K views

Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology
3:56

Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology

2.4K views

Bone Marrow Harvest from Mouse Hind Limb
3:38

Bone Marrow Harvest from Mouse Hind Limb

10.7K views

Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
2:57

Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow

4.4K views

Culturing and Harvesting of HSPC Clones: A Method to Obtain the Singly-Sorted HSPC Clones
5:21

Culturing and Harvesting of HSPC Clones: A Method to Obtain the Singly-Sorted HSPC Clones

1.3K views

Fluorescently Labeled T-ALL Cells Isolation: A Method to Isolate T-ALL Cells from Adult Zebrafish
4:43

Fluorescently Labeled T-ALL Cells Isolation: A Method to Isolate T-ALL Cells from Adult Zebrafish

1.2K views

Side Population Assay: A Method to Isolate Side Population of Tumor Cells from Zebrafish
4:31

Side Population Assay: A Method to Isolate Side Population of Tumor Cells from Zebrafish

1.9K views

Chromatin Shearing: A Method to Generate Chromatin Fragments Using Enzymatic Digestion
4:57

Chromatin Shearing: A Method to Generate Chromatin Fragments Using Enzymatic Digestion

1.3K views

In vitro techniques & assays

10 Videos

43 Minutes

Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
3:22

Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells

1.1K views

XTT Assay: A Colorimetric Assay to Assess Cell Viability and Mitochondrial Activity
3:20

XTT Assay: A Colorimetric Assay to Assess Cell Viability and Mitochondrial Activity

7.3K views

Bromodeoxyuridine Pulse Labelling Assay: A Technique to Measure Cell Proliferation
3:59

Bromodeoxyuridine Pulse Labelling Assay: A Technique to Measure Cell Proliferation

2.9K views

2D Co-culture of Leukemic Cells with BMSCs: A Method to Obtain Subpopulations of Leukemic Cells
3:27

2D Co-culture of Leukemic Cells with BMSCs: A Method to Obtain Subpopulations of Leukemic Cells

1.4K views

Preparation of Subcellular Fractions from Leukemic Cells: A Method to Generate Cellular and Nuclear Fractions from Leukemic Cells
4:25

Preparation of Subcellular Fractions from Leukemic Cells: A Method to Generate Cellular and Nuclear Fractions from Leukemic Cells

1.5K views

Extracellular Flux Assay: A Method for Measuring Metabolic Profile of Cells
4:39

Extracellular Flux Assay: A Method for Measuring Metabolic Profile of Cells

2K views

Chromatin Immunoprecipitation Assay: A Technique to Study Protein-DNA Interactions in Cells
6:29

Chromatin Immunoprecipitation Assay: A Technique to Study Protein-DNA Interactions in Cells

1.5K views

Retroviral-Mediated Transduction: A Method to Introduce Target Gene into Cancer Cells
4:59

Retroviral-Mediated Transduction: A Method to Introduce Target Gene into Cancer Cells

3.3K views

Methyl Cellulose-Based CFU Assay: A Method to Determine the Effect of Anti-cancer Compounds on Colony Formation in Leukemic Cells
3:31

Methyl Cellulose-Based CFU Assay: A Method to Determine the Effect of Anti-cancer Compounds on Colony Formation in Leukemic Cells

2.4K views

Extracellular Purine Metabolism: An HPLC-based Assay to Measure Adenosine Generation and Metabolism in Leukemic Cells
5:01

Extracellular Purine Metabolism: An HPLC-based Assay to Measure Adenosine Generation and Metabolism in Leukemic Cells

1.5K views

Pancreatic Cancer

21 Videos

100 Minutes

Assays & techniques

16 Videos

75 Minutes

DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
4:11

DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue

1.7K views

Imaging Calcium Dynamics in Pancreatic Cells: A Technique to Study Real-time Changes in Cytosolic Calcium Concentration in Pancreatic Islet
5:10

Imaging Calcium Dynamics in Pancreatic Cells: A Technique to Study Real-time Changes in Cytosolic Calcium Concentration in Pancreatic Islet

1.6K views

Immunofluorescent Staining of Spheroids: A Technique to Analyze Spheroids for Expression of Intra- and Extracellular Antigen Expression
4:29

Immunofluorescent Staining of Spheroids: A Technique to Analyze Spheroids for Expression of Intra- and Extracellular Antigen Expression

9.1K views

Bioluminescent Orthotopic Pancreatic Cancer Mouse Model: A Non-invasive Technique to Monitor Cancer Progression in Mice
5:43

Bioluminescent Orthotopic Pancreatic Cancer Mouse Model: A Non-invasive Technique to Monitor Cancer Progression in Mice

2.2K views

Ex Vivo T Cell Stimulation in the Pancreatic Tumor: A Method to Increase Cytokine Production in T Cells
5:00

Ex Vivo T Cell Stimulation in the Pancreatic Tumor: A Method to Increase Cytokine Production in T Cells

1.3K views

Extracellular Flux Assay in 3D Spheroids: A Method for Measuring the Metabolic Activity of Pancreatic Tumor Spheroids
5:39

Extracellular Flux Assay in 3D Spheroids: A Method for Measuring the Metabolic Activity of Pancreatic Tumor Spheroids

1.1K views

Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse
5:04

Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse

1.7K views

Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
3:33

Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging

1.4K views

Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models
3:59

Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models

2.1K views

Irreversible Electroporation: A Technique for Ablating Pancreatic Tumor in Mouse
3:50

Irreversible Electroporation: A Technique for Ablating Pancreatic Tumor in Mouse

1.3K views

Intracardiac Delivery of Viral Vectors: A Technique to Inject Viral Vectors Intracardially to Study Pancreatic Metastasis in Murine Model
4:38

Intracardiac Delivery of Viral Vectors: A Technique to Inject Viral Vectors Intracardially to Study Pancreatic Metastasis in Murine Model

4.1K views

Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
5:08

Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo

1.6K views

Sphere Formation Assay: An In Vitro Method to Identify Pancreatic Cancer Stem Cells and Screen Therapies
3:09

Sphere Formation Assay: An In Vitro Method to Identify Pancreatic Cancer Stem Cells and Screen Therapies

2.9K views

In Vitro Pancreatic Neuroplasticity Assay: Studying Changes in GI Tract Neurons Exposed to Healthy and Cancerous Human Pancreatic Tissue Extracts
4:24

In Vitro Pancreatic Neuroplasticity Assay: Studying Changes in GI Tract Neurons Exposed to Healthy and Cancerous Human Pancreatic Tissue Extracts

1K views

PTM Based Tumor Microenvironment Monitoring: A Method for Tracking Changes in ECM Rigidity Using Tumor Spheroids
6:26

PTM Based Tumor Microenvironment Monitoring: A Method for Tracking Changes in ECM Rigidity Using Tumor Spheroids

1K views

Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids
5:11

Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids

3.4K views

Cell isolation & culture

5 Videos

25 Minutes

Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
4:41

Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts

1.3K views

Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture
3:21

Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture

1.8K views

Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
7:21

Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells

4.2K views

Primary Acinar Cell Isolation: A Method to Isolate Acinar Cells from Mouse Pancreas
6:36

Primary Acinar Cell Isolation: A Method to Isolate Acinar Cells from Mouse Pancreas

1.9K views

PDAC Stem Cell Isolation: A Method to Isolate Cancer Stem Cells from Pancreatic Tumors
3:14

PDAC Stem Cell Isolation: A Method to Isolate Cancer Stem Cells from Pancreatic Tumors

1.2K views

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