Genetic Marker Detection
Genetic Marker Detection - Related Videos
08:45
Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers
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2025
In this video, we demonstrate the duplex digital PCR (ddPCR) technique — a modification of the traditional PCR technique that is useful in detecting two different genetic markers simultaneously. A single PCR reaction is partitioned into nanoliter-sized emulsified droplets that are independently amplified, and the detection of differently colored fluorescence amplification signals from the fraction of droplets is used to compute the initial concentration of the target sequences.
03:01
Detection of Target Enzyme Expression in Genetically Modified Bacterial Cells
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2025
Source: Kim, H. et. al., Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System. J. Vis. Exp. (2016)This video demonstrates a genetic screening method to detect enzyme expression using metagenomic fosmid libraries and chromogenic substrates. Flow cytometry identifies and isolates single cells producing colored products, enabling high-throughput selection of active enzyme-expressing cells.
12:45
Testing For Genetically Modified Foods
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Genetic modification of foods has been a controversial issue due to debated concerns over health and environmental safety. This experiment demonstrates technical understanding of how food DNA is genetically identified, allowing for educated decision making about the safety and potential dangers of using genetically modified organisms (GMOs) in food supplies. Polymerase Chain Reaction (PCR) is used to amplify food DNA...
08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
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Cited by 22 •
2017
This study implemented whole genome sequencing for analysis of mutations in genes conferring antifungal drug resistance in Candida glabrata. C. glabrata isolates resistant to echinocandins, azoles and 5-flucytosine, were sequenced to illustrate the methodology. Susceptibility profiles of the isolates correlated with presence or absence of specific mutation patterns in genes.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
FAQs
PCR supports marker detection by selectively amplifying a target DNA region, producing enough material for that region to be examined. The amplified sequence can then be compared with a reference sequence to determine whether the expected marker is present. In bioengineering, this makes PCR useful for checking engineered constructs and following genetic changes during strain development.
Nucleic acid hybridization indicates whether a target region binds a complementary sequence, whereas DNA sequencing reads the target region itself. Both approaches can support comparisons with reference sequences, but they provide different types of information. Choosing between them depends on whether the investigation requires target binding evidence or direct sequence information about a marker or variant.
A reference sequence supplies the standard against which an observed target region or variant is compared. This comparison helps determine whether a detected sequence matches an expected construct, differs from a reference, or identifies a genetic state of interest. In engineered systems, reference comparisons therefore support verification and assessment of genetic stability rather than detection alone.
Sequence specificity links the detection result to a particular DNA region rather than to genetic material in general. By targeting distinguishing sequences or variants, researchers can differentiate cells, organisms, engineered constructs, or biological states. This precision is especially important in bioengineering, where the result may be used to verify a modification or track a selected cell or trait.
A typical workflow begins by selecting the DNA region or variant that represents the marker and choosing a sequence-specific method. The target may then be amplified, bound by a complementary probe, or read through sequencing. Finally, the resulting information is compared with a reference sequence to support identification, construct verification, or evaluation of a genetic change.
Bioengineering projects use marker detection when they need to verify engineered constructs, track cells or traits, or monitor whether genetic material remains stable. The approach can also support strain development by identifying genetic features associated with desired performance. These uses connect molecular measurements with quality control and decisions about engineered biological systems.
Marker detection can provide evidence for tracking biological materials, evaluating genetic modifications, and identifying biomolecules associated with disease or production performance. It therefore contributes to more than confirming an engineered sequence. In research and biotechnology, the resulting information can support diagnostics, quality control, strain development, and assessment of how genetic features relate to biological or manufacturing outcomes.