Time-of-flight Secondary Ion Mass Spectrometry
Time-of-flight Secondary Ion Mass Spectrometry - Related Videos
07:24
Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry
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2019
Time-of-flight secondary ion mass spectrometry is applied to demonstrate the chemical mapping and corrosion morphology at the metal-paint interface of an aluminum alloy after being exposed to a salt solution compared with a specimen exposed to air.
11:44
Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
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Cited by 9 •
2016
A two-dimensional gas chromatography-time-of-flight mass spectrometry method is described for characterization of the aqueous fraction of bio-crude produced from hydrothermal liquefaction of algae. This protocol can also be employed to analyze the aqueous fraction of liquid products from fast pyrolysis, catalytic fast pyrolysis, catalytic deoxygenation and hydro-treating.
05:52
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
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Cited by 7 •
2024
An analytical workflow based on liquid chromatography, trapped ion mobility spectrometry, and time-of-flight mass spectrometry (LC-TIMS-ToF MS/MS) for high confidence and highly reproducible "bottom-up" analysis of histone modifications and identification based on principal parameters (retention time [RT], collision cross section [CCS], and accurate mass-to-charge [m/z] ratio).
08:51
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
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2025
Here, we present a detailed protocol for the release of O-glycans from mucins, and the subsequent desalting, permethylation, and analysis using MALDI-TOF mass spectrometry.
07:27
MALDI-TOF Mass Spectrometry
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2023
Matrix-assisted laser desorption ionization (MALDI) is a mass spectrometry ion source ideal for the analysis of biomolecules. Instead of ionizing compounds in the gaseous state, samples are embedded in a matrix, which is struck by a laser. The matrix absorbs the majority of the energy; some of this energy is then transferred to the sample, which ionizes as a result. Sample ions can then be identified using a time-of-flight analyzer (TOF). This video covers principles of MALDI-TOF, including...
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The focused primary ion beam transfers energy to the sample surface and ejects secondary ions from its outermost layers. Because the detected material originates near the surface, the resulting signal emphasizes interfacial chemistry rather than bulk composition. This makes the technique particularly relevant when bioengineered performance depends on surface coatings, biomolecules, or contaminants.
After emission, secondary ions travel through a vacuum analyzer, and their flight times are used to separate them according to mass-to-charge ratio. The resulting mass information supports identification of chemical components present at the analyzed surface. In bioengineering studies, this can help distinguish contributions from biomolecules, polymers, coatings, and unwanted contaminants.
Spatial mapping shows where chemical components are located across a surface, rather than reporting composition only as an overall average. This distinction can reveal whether biomolecules, polymers, or coatings are distributed uniformly or concentrated in particular regions. Such information helps connect local surface chemistry with cell-material interactions and tissue-interface behavior.
ToF-SIMS can provide information about both the identities and locations of chemical components within the outermost sample layers. Relevant signals may include biomolecules associated with tissue interfaces, polymer-based material components, surface coatings, and contaminants. Examining these categories together supports a more detailed assessment of how a bioengineered surface is chemically presented.
A typical workflow begins by directing a focused primary ion beam at the sample surface. The emitted secondary ions then enter a vacuum analyzer, where their flight times separate them by mass-to-charge ratio. The measured signals can subsequently be used to identify surface components and create maps showing their spatial distribution.
Researchers may choose this technique when they need surface-sensitive chemical information from biomaterials, tissue interfaces, or engineered surfaces. Its ability to identify and map components supports investigations of cell-material interactions, drug delivery systems, and surface coatings. It is especially useful when the location of a chemical component matters alongside its presence.
For drug delivery systems and engineered surfaces, the method can examine which chemical components are present at the exposed surface and where they occur. Mapping may help characterize polymers, coatings, biomolecules, or contaminants associated with the design. These observations provide surface-level evidence for evaluating how an engineered system presents its chemistry to its surroundings.