Tumor Vasculature Imaging
Tumor Vasculature Imaging - Related Videos
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
08:52
Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
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Cited by 32 •
2018
This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes.
09:03
Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
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Cited by 7 •
2014
We describe a matrigel plug assay to illustrate angiogenic potential of a pool of factors secreted by cancer cells using two complementary imaging modalities, ultrasound and endomicroscopy. The matrigel, an extracellular matrix (ECM)-mimic gel, is utilized to introduce the host (mouse) with angiogenic factors secreted to the conditioned media...
14:04
Viral Nanoparticles for In vivo Tumor Imaging
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Cited by 45 •
2012
Plant viral nanoparticles (VNPs) are promising platforms for applications in biomedicine. Here, we describe the procedures for plant VNP propagation, purification, characterization, and bioconjugation. Finally, we show the application of VNPs for tumor homing and imaging using a mouse xenograft model and fluorescence imaging.
12:09
A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
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Cited by 14 •
2013
We describe a novel in vivo imaging technique that couples fluorescent chimeric mice with intracranial windows and high-resolution 2-photon microscopy. This imaging platform aids studies of dynamic changes in brain tissue and microvasculature, at a single-cell level, following pathological insults and is adaptable to assess intracranial drug delivery and distribution.
FAQs
Vessel density, diameter, perfusion, permeability, and architectural irregularity provide complementary information. Density and diameter describe the vascular network, while perfusion indicates how blood moves through it. Permeability and abnormal architecture can reveal differences between tumor-associated vessels and more organized circulation. Together, these measurements help characterize tumor growth, disease progression, and changes produced by treatment.
Contrast agents make vascular behavior more distinguishable from surrounding tissue by highlighting circulation or vascular permeability. Their distribution can therefore add functional information to structural observations, helping researchers assess how blood reaches a tumor and how readily material passes through its vessels. This information is particularly useful when developing imaging probes or evaluating delivery systems.
These modalities emphasize different aspects of the vascular system. Magnetic resonance imaging and computed tomography can support visualization of vascular structure or circulation, whereas ultrasound and optical techniques can also provide information about blood flow or vessel behavior, depending on the implementation. The appropriate choice depends on whether the study prioritizes architecture, perfusion, permeability, or another measurable vascular feature.
Tumor-associated vessels may be evaluated not only by their number but also by their abnormal architecture. Irregular patterns can provide additional evidence about how the vascular network relates to tumor growth and progression. Measuring these structural features alongside diameter, density, and perfusion gives bioengineers a broader basis for designing vascularized models and assessing whether an intervention changes the tumor blood supply.
A study generally begins by selecting an imaging modality suited to the vascular feature of interest, followed by image acquisition with or without a contrast agent when circulation or permeability must be emphasized. Researchers then quantify features such as vessel density, diameter, perfusion, or architecture and compare those measurements across conditions. This workflow supports evaluation of tumor status or treatment response.
Bioengineers apply these measurements when designing vascularized tissue models, drug-delivery systems, and probes that target angiogenic vessels. Imaging data can indicate whether a model reproduces relevant vascular characteristics or whether a delivery strategy reaches the intended tumor-associated circulation. The same measurements also help guide development of approaches intended to disrupt tumor blood supply.
Repeated assessment of vessel density, diameter, perfusion, permeability, or architectural abnormality can reveal how a tumor vascular network changes during treatment. A shift in these measurements may provide evidence that an intervention affects blood supply or vascular behavior, complementing observations of tumor progression. This makes imaging useful for comparing treatment responses and guiding therapies directed at tumor vasculature.
Imaging probes can be designed to target angiogenic vessels, allowing vascular features to be highlighted more selectively within a tumor environment. Their development depends on understanding which vascular properties, such as permeability or abnormal architecture, distinguish the vessels of interest. In bioengineering, such probes connect molecular targeting with measurable imaging outcomes for studying tumor progression or therapy response.