Yfp-mcherry Reporter
Yfp-mcherry Reporter - Related Videos
10:03
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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Cited by 11 •
2012
Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.
07:16
The In Vitro Single Oocyte Reporter Assay: A Technique to Study Target mRNA Translation Regulation During In Vitro Oocyte Maturation
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2025
In this video, we demonstrate that in the presence of a phosphodiester inhibitor, the oocyte cell cycle is arrested at prophase-I, during which transcribed mRNAs are stored in the absence of translation. After the release of the inhibitor, oocyte maturation resumes, and the cells enter metaphase. The signal of reporter mRNA fused with the target gene in metaphase-I indicates the activation of translational machinery and protein expression.
12:02
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
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Cited by 1 •
2020
Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).
01:24
Data Reporting and Recording
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2023
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
04:25
Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene
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2025
This video demonstrates an in vivo assay in bacteria to study the interaction of RNA-binding proteins (RBPs) with RNA. The bacterial cells are transformed with two plasmid constructs — a binding-site plasmid encoding an mRNA containing a fluorescent reporter gene downstream of an RBP-binding site — while an RBP plasmid expresses the RBP proteins under the control of an inducer. Upon inducer-mediated increase in RBP production, the RBPs bind to the mRNA, resulting in translational repression of...
FAQs
YFP and mCherry are distinguished through their different excitation and emission properties. In practice, microscopy can treat each fluorescent signal as a separate readout rather than as one combined intensity value. This separation lets an investigator compare where each signal appears and when it changes, supporting analysis of coordinated or contrasting molecular behavior within the same specimen.
A time-resolved comparison can show whether two signals change together, appear at different times, or occupy different regions of a cell. Because both readouts come from the same living specimen, the analysis can connect molecular events more directly than observations made in separate samples, while preserving spatial and temporal information.
The relevant interpretation depends on the biological question. In promoter-activity work, changes in fluorescence can be followed as an expression readout; in protein-localization work, the signal distribution can be tracked; for cellular behavior, changes across the specimen can be monitored. The paired outputs permit these observations alongside the other fluorescent signal.
Using two distinguishable signals adds a comparison dimension to microscopy. A single fluorescent readout can show one distribution or change, whereas YFP-mCherry enables researchers to examine two patterns in parallel and relate their locations or timing. This is particularly useful when the question concerns whether molecular events occur in the same specimen.
Analysis begins by observing living cells with microscopy and distinguishing the YFP and mCherry signals through their separate excitation and emission properties. Researchers then compare each signal’s distribution or timing, depending on the experiment, and assess how the patterns relate. The resulting observations can connect fluorescence changes with expression, localization, or cellular behavior.
It is useful when an experiment needs simultaneous information about two fluorescently marked features in living cells. Examples supported by this approach include monitoring promoter activity, tracking tagged-protein localization, following cellular dynamics, and examining relationships between molecular events. The design can reduce dependence on separate samples or additional labeling procedures.
Researchers can compare the spatial distribution and timing of the YFP and mCherry signals within the same specimen. If the patterns coincide, differ, or change in sequence, those observations provide a basis for relating the monitored events. The reporter therefore supports integrated analysis rather than requiring each event to be examined independently.