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Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
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Neuroscience
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JoVE Journal Neuroscience
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

DOI:
10.3791/3848-v

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10:03 min

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September 23, 2012

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, , ,
1Department of Biomedical Engineering,Yale University, 2Department of Biomedical Engineering,Louisiana Tech University

Chapters

  • 00:00Title
  • 03:08Animal Perfusion and Whole Mouse Brain Clearing
  • 06:57Microscope Setup
  • 08:12Representative Results and Conclusion

Summary

Automatic Translation

Automatic Translation

Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.

Tags

Multiphoton MicroscopyCleared Mouse BrainYFP ExpressionIntrinsic FluorescenceSecond Harmonic Generation (SHG)Optically ClearingMethanol DehydrationBenzyl Alcohol:benzyl Benzoate (BABB)Fluorescent SignalOptical Clearing ProtocolsEthanol Graded SeriesFluorescent ProteinsMultiphoton ImagingHigh-resolution ImagesNeurons

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