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Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
 

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Article DOI: 10.3791/3848
September 23rd, 2012

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Summary September 23rd, 2012

Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.

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